American Genetics Lab

THINGS TO KNOW
ABOUT PCR TEST

BRIEF INTRO ABOUT PCR
TESTING

The abbreviation of PCR stands for polymerase chain reaction and also called molecular test. It’s a test that looks for genetic material from a certain organism and itt is very precise and sensitive.

HOW DOES PCR WORKS?

DNA DENATURES

To use PCR to amplify a segment of DNA, the sample is heated until the DNA denatures, or separates into two single-stranded DNA pieces

DNA REPLICATION

Then, using the original strands as templates, an enzyme called “Taq polymerase” synthesizes – or creates – two new strands of DNA.

DNA ELONGATION

After that, each of these strands can be used to make two more copies, and so on. Denaturing and synthesizing new DNA is repeated 30 or 40 times,

WHAT DOES PCR DO?

PCR is a very sensitive
technology for rapidly amplifying a specific DNA fragment.

PCR produces billions of copies of a specific DNA .fragment or gene, allowing gene sequences to be detected and identified using visual approaches based on size and charge

With real-time PCR techniques, modified versions of PCR have permitted
quantitative measures Quantitative PCR (qPCR), also known as real-time PCR or quantitative real-time PCR, is a PCR-based technique that combines the amplification of a target DNA sequence with the determination of that DNA species' concentration in a reaction.
of gene expression.

want to get PCR test for 

COVID-19

Origin of PCR Test

The "chain termination method," often known as ,
Sanger sequencing The "chain termination method," often known as Sanger sequencing, is a method for determining the nucleotide sequence of DNA.
is a method for determining the nucleotide sequence of DNA. The Sanger Sequence was created in 1977 by two-time Nobel Laureate Frederick Sanger and his collaborators, hence the name.
A complementary DNA primer to the template DNA (to be sequenced) is utilized as a starting point for DNA synthesis in Sanger sequencing..

TESTING FOR

COVID-19 VIA PCR

HOW DOES THE PCR
TEST WORK FOR
COVID-19?

HOW DOES THE PCR
TEST WORK FOR
COVID-19?

HOW DOES THE PCR
TEST WORK FOR
COVID-19?

• In a lab test, swabs from the nose and throat.
• …sample used to detect the presence of the SARS-CoV-2 virus.

HOW DOES THE PCR
TEST WORK FOR
COVID-19?

HOW DOES THE PCR
TEST WORK FOR
COVID-19?

• In a lab test, swabs from the nose and throat.
• …sample used to detect the presence of the SARS-CoV-2 virus.

HOW DOES THE PCR
TEST WORK FOR
COVID-19?

HOW DOES THE PCR
TEST WORK FOR
COVID-19?

HOW DOES THE PCR
TEST WORK FOR
COVID-19?

• In a lab test, swabs from the nose and throat.
• …sample used to detect the presence of the SARS-CoV-2 virus.

HOW DOES THE PCR
TEST WORK FOR
COVID-19?

HOW DOES THE PCR
TEST WORK FOR
COVID-19?

• In a lab test, swabs from the nose and throat.
• …sample used to detect the presence of the SARS-CoV-2 virus.

HOW DOES THE PCR
TEST WORK FOR
COVID-19?

HOW DOES THE PCR
TEST WORK FOR
COVID-19?

HOW DOES THE PCR
TEST WORK FOR
COVID-19?

WHY YOU NEED TO KNOW ABOUT SARS-CoV-2 IN PCR TEST?

The SARS-CoV-2 virus causes Coronavirus Disease (COVID-19), an infectious disease.

For the SARS-CoV-2 virus, there are three types of tests:
molecular tests, antigen tests, and antibody tests. The molecular and antigen testing are the ones that are used to diagnose COVID-19. After complete recovery from COVID-19, antibody testing, often known as serology testing, is performed.

RAPID ANTIGEN TESTING Vs RT-PCR

COVID-19 rapid antigen tests are used to detect antigens or viral proteins in the body that trigger an immune response and indicate viral infection.

COVID-19 test kits, on the other hand, use real-time reverse transcription-polymerase chain reaction (RT-PCR) to detect genetic material. As a result, RT-PCR remains the diagnosis since it more correctly confirms the presence of coronavirus and whether or not a person is infected.

DIFFERENT TYPES OF PCR:

(RT-PCR) Reverse Transcriptase PCR

Real-time PCR

Quantitative real time PCR (Q-RT PCR)

Multiplex PCR

Nested PCR

Long-range PCR

Single-cell PCR

Fast-cycling PCR

Methylation-specific PCR (MSP)

Hot start PCR

High-fidelity PCR

WHY CHOOSE PCR TEST?

In medical and biological research labs, PCR is a common tool. It’s utilized in the early stages of DNA

processing for sequencing, to detect the presence or lack of a gene to aid in pathogen identification during producing forensic DNA profiles from small quantities of DNA, and during infection

PANDEMIC

Having a healthy snack on hand can help you add nutrients to your diet and feel less hungry when you sit down to a meal…;

COVID-19 TESTING

Having a healthy snack on hand can help you add nutrients to your diet and feel less hungry when you sit down to a meal…;

PANDEMIC

Having a healthy snack on hand can help you add nutrients to your diet and feel less hungry when you sit down to a meal…;

PROS & CONS

OF pCR TESTING

Multiple targets are tested in one sample, increasing diagnostic yield

Due to the use of selective amplification of one target over another, false-negative results were obtained.

Standarized and accurate
testing

Commercial kits and
instrumentsare very
expensive۔

WHAT WILL BE THE COST OF PCR Test

• Same Day Results PCR $149.00

• Antigen Test (Rapid Test) $79

Covid testing lab contact No

4731 W Atlantic Ave Suite B-21, Delray Beach, FL 33445

Covid testing lab contact No

Covid testing lab contact No

Email

testing@americangeneticslab.com

WHAT DOES PCR DO?

PCR is a very sensitive technology for rapidly amplifying a specific DNA fragment.

PCR produces billions of copies of a specific DNA fragment or gene, allowing gene sequences to be detected and identified using visual approaches based on size and charge.

With real-time PCR techniques, modified versions of PCR have permitted                                                               of gene expression.

quantitative measures

Quantitative PCR (qPCR), also known as real-time PCR or quantitative real-time PCR, is a PCR-based technique that combines the amplification of a target DNA sequence with the determination of that DNA species' concentration in a reaction

FAQ

WHAT IS MYCOPLASMA PCR TESTING?

With a detection limit of 10 CFU/mL, the EZ-PCRTM Mycoplasma Detection Kit is a highly sensitive and specific PCR assay developed to test for the presence of over 90 species of Mycoplasma, Acholeplasma, and Spiroplasma in cell cultures.

WHAT IS A LEGIONELLA PCR TEST?

A positive PCR result for the presence of a specific sequence discovered within the Legionella 5S rRNA gene shows the presence of Legionella species DNA in the specimen, which could be due to Legionella infection or environmental/water Legionella DNA.

THINGS TO KNOW ABOUT PCR TEST

BRIEF INTRO ABOUT PCR TESTING

The abbreviation PCR stands for polymerase chain reaction and also called molecular test. It's a test that looks for genetic material from a certain organism. PCR is also known as "molecular photocopying" and is very precise and sensitive. Primers are short stretches of that are used to
DNA DNA is the genetic substance that contains all of the information about a living thing's appearance and function. Human DNA, for example, regulates the color of the eyes and the function of the lungs. Each bit of data is stored in a distinct section of the DNA. Genes are the names for these sections.
selectively amplify a certain sequence..

HOW DOES PCR WORKS?

DNA DENATURES

To use PCR to amplify a segment of DNA, the sample is heated until the DNA denatures, or separates into two single-stranded DNA pieces

DNA REPLICATION

Then, using the original strands as templates, an enzyme called “Taq polymerase” synthesizes – or creates – two new strands of DNA.

DNA ELONGATION

After that, each of these strands can be used to make two more copies, and so on. Denaturing and synthesizing new DNA is repeated 30 or 40 times,

WHAT DOES PCR DO?

With real-time PCR techniques, modified versions of PCR have permitted
quantitative measures Quantitative PCR (qPCR), also known as real-time PCR or quantitative real-time PCR, is a PCR-based technique that combines the amplification of a target DNA sequence with the determination of that DNA species' concentration in a reaction.
of gene expression.

Want to get PCR test for

COVID-19

Origin of PCR Test

The "chain termination method," often known as ,
Sanger sequencing The "chain termination method," often known as Sanger sequencing, is a method for determining the nucleotide sequence of DNA.
is a method for determining the nucleotide sequence of DNA. The Sanger Sequence was created in 1977 by two-time Nobel Laureate Frederick Sanger and his collaborators, hence the name.
A complementary DNA primer to the template DNA (to be sequenced) is utilized as a starting point for DNA synthesis in Sanger sequencing..

TESTING FOR COVID-19 VIA PCR

HOW DOES THE PCR TESTING
WORK FOR COVID-19?

• In a lab test, swabs from
the nose and throat.
• …sample used to detect
the presence of the SARS-
CoV-2 virus.

WHAT CAN PCR TESTING
ASSURE YOU?

• A COVID-19 PCR test is a
diagnostic tool for those
who have been infected with
SARS-CoV-2, the virus that
cause COVID-19.

WHEN IS THE PCR TEST USED
TO DETECT COVID- 19?

• From a week to a few
months with the infection of
SARS-CoV-2 virus.
• If a patient is tested before
the body has had time to
generate antibodies, he or
she may test negative.

 

HOW ACCURATE PCR
TESTING FOR COVID-19?

• PCR is a well-established
method that provides a very
sensitive and accurate result.

TESTING FOR COVID-19 VIA PCR

HOW DOES THE PCR TESTING WORK FOR COVID-19?
• In a lab test, swabs from
the nose and throat.
•sample used to detect
the presence of the SARS-
CoV-2 virus.
WHEN IS THE PCR TEST USED
TO DETECT COVID- 19?

• From a week to a few
months with the infection of
SARS-CoV-2 virus.
• If a patient is tested before
the body has had time to
generate antibodies, he or
she may test negative.
WHAT CAN PCR TESTING
ASSURE YOU?

• A COVID-19 PCR test is a
diagnostic tool for those
who have been infected with
SARS-CoV-2, the virus that
cause COVID-19.
HOW ACCURATE PCR
TESTING FOR COVID-19?

• PCR is a well-established
method that provides a very
sensitive and accurate
result.

PCR'S APPLICATIONS

WHY YOU NEED TO KNOW ABOUT SARS-CoV-2 IN PCR TEST?

The SARS-CoV-2 virus causes Coronavirus Disease (COVID-19), an infectious disease.
For the SARS-CoV-2 virus, there are three types of tests: molecular tests,
antigen The proteins produced by a virus are known as viral proteins. Viruses encode extremely few of their own genes because they need so much of their host's cellular machinery to maintain their life cycle; viral proteins are therefore mostly structural components of the viral envelope and capsid..
tests, and antibody tests. The molecular and antigen testing are the ones that are used to diagnose COVID-19. After complete recovery from COVID-19, antibody testing, often known as serology testing, is performed.

RAPID ANTIGEN TESTING AND RT-PCR

COVID-19 rapid antigen tests are used to detect antigens or
Viral protein The proteins produced by a virus are known as viral proteins. Viruses encode extremely few of their own genes because they need so much of their host's cellular machinery to maintain their life cycle; viral proteins are therefore mostly structural components of the viral envelope and capsid..
in the body that trigger an immune response and indicate viral infection.
COVID-19 test kits, on the other hand, use real-time reverse transcription-polymerase chain reaction (RT-PCR) to detect
genetic material. The hereditary ingredient in the cell is genetic material. It contains all of an organism's particular information. It's also known as DNA (deoxyribonucleic acid) or RNA (ribonucleic acid) (ribonucleic acid)
As a result, RT-PCR remains the diagnosis since it more correctly confirms the presence of coronavirus and whether or not a person is infected.

KEY FEATURES OF PCR:

PCR uses
Taq polymerase The proteins produced by a virus are known as viral proteins. Viruses encode extremely few of their own genes because they need so much of their host's cellular machinery to maintain their life cycle; viral proteins are therefore mostly structural components of the viral envelope and capsid..
a thermostable DNA polymerase, and DNA primers prepared especially for the DNA area of interest..

DIFFERENT TYPES OF PCR

WHY CHOOSE PCR TEST?

In medical and biological research labs, PCR is a common tool. It’s utilized in the early stages of DNA processing for sequencing, to detect the presence or lack of a gene to aid in pathogen identification during producing forensic DNA profiles from small quantities of DNA, and during infection

IN YOUR NEARBY AREA

WHAT WILL BE THE COST OF PCR Test

You would have probably heard of GERD, or gastroesophageal disease, which is a common inflammatory disease that causes heartburn and a burning feeling in the base of your throat. However, you may not know why GERD happens; when the muscle that connects the esophagus to the stomach malfunctions, GERD develops. The lower esophageal sphincter
Read More

Why Knowing Medical History? There are various compelling reasons for families to communicate their medical histories. Some diseases, for example, run in families. Diabetes, heart disease, several malignancies, and arthritis are among them. In addition, high blood pressure, stroke, and life expectancy are all affected by genetic factors. You don't have to be diagnosed with
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What Are DNA Medical Tests? Each person has a unique genome, which consists of the DNA of the individual. An essential step in genetic testing is to talk to your doctor, medical geneticist, or genetic counselor about what the results will do. If genetic testing does not lead to a diagnosis, but a genetic cause
Read More

Pendemic

Having a healthy snack on hand can help you add nutrients to your diet and feel less hungry when you sit down to a meal. Here are 29 healthy snacks…

Covid-19 testing

Having a healthy snack on hand can help you add nutrients to your diet and feel less hungry when you sit down to a meal. Here are 29 healthy snacks…

Covid-19 testing

Having a healthy snack on hand can help you add nutrients to your diet and feel less hungry when you sit down to a meal. Here are 29 healthy snacks…

Cons

Multiple targets are tested in one sample, increasing diagnostic yield

Due to the use of selective amplification of one target over another, false-negative results were obtained.

Commercial kits and instrumentsare very
expensive۔

Standarized and accurate testing

PROS

CONS

PROS & CONS OF

PCR TESTING

Frequently Asked Questions

WHAT IS MYCOPLASMA PCR TESTING?

With a detection limit of 10 CFU/mL, the EZ-PCRTM Mycoplasma Detection Kit is a highly sensitive and specific PCR assay developed to test for the presence of over 90 species of Mycoplasma, Acholeplasma, and Spiroplasma in cell cultures.

WHAT IS A LEGIONELLA PCR TEST?

A positive PCR result for the presence of a specific sequence discovered within the Legionella 5S rRNA gene shows the presence of Legionella species DNA in the specimen, which could be due to Legionella infection or environmental/water Legionella DNA.

WHO DISCOVERED PCR?

Kary Banks Mullis was a biochemist from the United States. He shared the Nobel Prize in Chemistry in 1993 with Michael Smith for his contribution in the discovery of the polymerase chain reaction technique, and he was also awarded the Japan Prize in the same year.

PANDEMIC

Having a healthy snack on hand can help you add nutrients to your diet and feel less hungry when you sit down to a meal…;

PANDEMIC

Having a healthy snack on hand can help you add nutrients to your diet and feel less hungry when you sit down to a meal…;

PANDEMIC

Having a healthy snack on hand can help you add nutrients to your diet and feel less hungry when you sit down to a meal…;

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